A synthetic gene increases TGFβ3 accumulation by 75-fold in tobacco chloroplasts enabling rapid purification and folding into a biologically active molecule
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منابع مشابه
Over Expression of Biologically Active Interferon Beta Using Synthetic Gene in E. coli
In this study, our previously reported novel synthetic gene encoding 166 residues of interferon-? was used for an efficient expression of IFN-?. The synthetic gene was cloned into pET21a expression vector and transferred into E. coli. Recombinant protein was over-expressed in the E. coli. Identity of the recombinant protein was confirmed by western blot analysis. The recombinant protein was bio...
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ژورنال
عنوان ژورنال: Plant Biotechnology Journal
سال: 2011
ISSN: 1467-7644
DOI: 10.1111/j.1467-7652.2011.00619.x